GUCY2C Signaling Opposes the Acute Radiation-Induced GI Syndrome.
MouseRadiationC57BL/6 wild type, Gucy2c germline knockout backcrossed at least 14 generations onto C57BL/6, and villin-CreERT2 crossed p53 floxed mice on mixed FVB.129 and C57BL/6 backgroundsMale and femalen = At least 3 animals per group; exact group sizes per experim…Oral gavage10 micrograms per mouse in 200 microlitres PBS (peptide sto…Daily for 14 days before and 14 days after irradiation
Model: Acute radiation-induced gastrointestinal syndrome from caesium-137 gamma irradiation at about 70 cGy/min, 15 Gy total body irradiation or 18 Gy subtotal abdominal irradiation with bone marrow shielding, across a dose range of 8 to 25 Gy
Deleting GUCY2C worsened radiation-induced gastrointestinal syndrome with earlier death, more diarrhoea, weight loss, intestinal bleeding, debilitation and crypt loss including new colonic vulnerability. Receptor and hormone expression persisted after lethal irradiation. Oral ST reduced diarrhoea,…
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