Granulocyte colony-stimulating factor blockade enables dexamethasone to inhibit lipopolysaccharide-induced murine lung neutrophils.
MouseEndotoxin/LPSBALB/cMalen = n = 4 to 8 mice per group for BAL endpoints and n = 6 for B…Anti-G-CSF antibody intraperitoneal in 100 microlitres PBS; dexamethasone intraperitoneal in 100 microlitres PBS; LPS intratracheal in 100 microlitres PBSAnti-G-CSF antibody 250 micrograms/kg; dexamethasone 2.5 mg…Both dexamethasone and anti-G-CSF antibody given 6 hours after the LPS challeng…
Model: Intratracheal lipopolysaccharide (1 mg/kg) induced acute lung injury
Dexamethasone alone suppressed BAL TNF and IL-6 but did not reduce LPS-induced G-CSF, BAL neutrophils, BAL total protein or lung injury, and increased airway neutrophil numbers at 24 hours. Adding the anti-G-CSF neutralising antibody allowed dexamethasone to lower BAL G-CSF and neutrophil numbers a…
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