Pullulan hydrogels improve mesenchymal stem cell delivery into high-oxidative-stress wounds.
MouseWoundFVB/NJ wildtype; transgenic firefly luciferase/eGFP reporter mice on FVB background as cell donorsFemaleSubcutaneous implantation of the cell-seeded hydrogel scaffold beneath the wound, compared with peri-wound subcutaneous injection of cells in PBS2.5 x 10^n mesenchymal stem cells delivered per wound site,…Immediately following injury (day 0)
Model: Two models were used: dorsal full-thickness transverse incisions (1 cm) with subcutaneous scaffold or cell implantation, and 3 mm full-thickness punch biopsy wounds created in the distal third of an ischaemic peninsular skin flap. In vitro oxidative stress was applied with hydrogen peroxide.
Hydrogel-seeded mesenchymal stem cells showed markedly better survival than plated cells after hydrogen peroxide exposure (12.4 plus or minus 7.2 versus 37.8 plus or minus 19.9 per cent non-viable at the lower concentration, p = 0.015; 45.11 plus or minus 18.3 versus 87.8 plus or minus 5.3 per cent…
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