Parabrachial Pituitary Adenylate Cyclase-Activating Polypeptide Activation of Amygdala Endosomal Extracellular Signal-Regulated Kinase Signaling Regulates the Emotional Component of Pain.
MixedCrushPeripheral-nerve crushAdult Sprague-Dawley rats; PACAP-EGFP BAC transgenic miceMalen = 5 to 8 animals per group per experimentBilateral intra-amygdalar infusion into the central nucleus via 22 GA cannulae at AP -2.6, ML 4.5, DV -7.2 mm from bregma, at 0.5 microlitre/minPD98059 20 micromolar and Pitstop 2 30 micromolar by intra-…Acute infusion 14 days after chronic constriction injury, approximately 1 h bef…
Model: Partial sciatic nerve chronic constriction injury using loose 4-0 chromic gut ties placed proximal to the sciatic trifurcation
Chronic constriction injury increased PACAP expression along the spino-parabrachio-amygdaloid tract, reduced open field centre entries by more than half, shortened ipsilateral thermal latency by 40 to 50 per cent and increased CeLC and BNST pERK cell counts. Bilateral central amygdala PACAP(6-38) b…
Extraction: extract · Subtype source: rule · Supplied extraction confidence: 0.88. Machine-extracted fields require source verification.