In Situ Bioprinting of Autologous Skin Cells Accelerates Wound Healing of Extensive Excisional Full-Thickness Wounds.
MixedWoundOutbred athymic nude (Nu/nu) mice; specific pathogen free Yorkshire pigsFemale (mice); not stated for pigsn = Mouse: 36 mice in three groups of 12, with n = 4 per time p…Topical in situ bioprinting directly onto the excised wound bed, dermal layer followed by epidermal layerMouse: fibroblast layer then keratinocyte layer at a 1:2 ce…Immediately after excision at the time of surgery; the keratinocyte layer was p…
Model: Mouse: single 3 x 2.5 cm full-thickness excisional dorsal skin defect, approximately 50% of skin surface area. Pig: four 10 x 10 cm full-thickness excisional wounds on the dorsum, excised to the panniculus carnosus
Bioprinted autologous fibroblasts and keratinocytes accelerated wound closure (mouse wounds 66% of original area at 1 week versus 95% in controls, closed by 3 weeks versus 5 weeks; pig autologous wounds fully closed by week 5, roughly a 3 week acceleration), reduced contraction by about 50% and acc…
Extraction: extract · Supplied extraction confidence: 0.85. Machine-extracted fields require source verification.