A special population of regulatory T cells potentiates muscle repair.
MouseChemical/toxinC57BL/6, C57BL/10ScSn, C57BL/10ScSn-Dmd(mdx), RAG1-deficient, Foxp3-DTR, Foxp3-IRES-GFP and dysferlin-deficient miceIntramuscular for the first amphiregulin dose then intraperitoneal; intraperitoneal for IL-2 complexes, anti-CD25 and diphtheria toxinAmphiregulin 1 ng per muscle intramuscularly then 7 microgr…Amphiregulin given intramuscularly together with cardiotoxin at day 0; diphther…
Model: Acute skeletal muscle injury by intramuscular cardiotoxin (Naja mossambica mossambica) injection into tibialis anterior, gastrocnemius and quadriceps; cryoinjury of tibialis anterior by dry ice exposure; chronic dystrophic muscle damage in mdx and dysferlin-deficient mice
Punctual regulatory T cell ablation increased the cellular infiltrate, blocked the myeloid switch from Ly6c high to Ly6c low, produced disorganised histology with fibrosis and fewer centrally nucleated fibres, and reduced satellite cell colony-forming capacity. Expansion of regulatory T cells with…
Extraction: extract · Supplied extraction confidence: 0.8. Machine-extracted fields require source verification.