TRPM7 in CHBP-induced renoprotection upon ischemia reperfusion-related injury.
MouseIschaemia-reperfusionRenalBALB/c (in vivo); TCMK-1 (mouse) and HK-2 (human) renal tubular epithelial cell lines used in vitroMalen = In vivo: n=6 mice per group per time point; in vitro: at le…Intraperitoneal injection (CHBP, in vivo); tail vein injection (TRPM7 siRNA, in vivo); direct addition to culture medium (in vitro)CHBP 8 nmol/kg (in vivo, intraperitoneal) or 30 nM (in vitr…CHBP administered at the onset of reperfusion; TRPM7 siRNA administered 2 h bef…
Model: Renal ischemia-reperfusion injury: bilateral renal pedicle clamping for 30 min ischemia followed by reperfusion for 12 h to 7 days in mice; complemented by in vitro hypoxia (12-24 h) followed by 2-24 h reoxygenation in TCMK-1/HK-2 cells
CHBP reversed the ischemia-reperfusion-induced upregulation of TRPM7 expression and TRPM7-like current, and reduced downstream inflammation (HMGB1) and apoptosis (caspase-3, Bax/Bcl-2 ratio) in both hypoxia-reoxygenation-treated tubular cells and mouse IR kidneys. TRPM7 knockdown by siRNA similarly…
Extraction: extract · Subtype source: rule · Supplied extraction confidence: 0.85. Machine-extracted fields require source verification.