Neural stem cell mediated recovery is enhanced by Chondroitinase ABC pretreatment in chronic cervical spinal cord injury.
MouseSpinal cord injuryContusionC57BL/6 (wild type, Jackson Laboratories)Femalen = 70 adult mice in total: 10 sham, 60 clip-contusion; behavio…ChABC intrathecal via catheter and osmotic mini-pump; iPS-NSC intraparenchymal stereotactic injection; ciclosporin A subcutaneousChABC 3.64 mU/mL in artificial cerebrospinal fluid infused…Chronic phase: ChABC infusion started 7 weeks after injury; iPS-NSC transplante…
Model: C6/7 laminectomy followed by modified aneurysm clip (FEJOTA mouse clip) contusion-compression at a closing force of 6.5 g for 40 seconds; sham animals received laminectomy only
ChABC pretreatment increased iPS-NSC graft survival from 2.44 plus or minus 1.04 per cent to 7.88 plus or minus 1.60 per cent (p = 0.017) and reduced CSPG and GFAP labelling. Only the combined ChABC plus iPS-NSC group showed significant recovery of forelimb grip strength versus control (mean differ…
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