EphB3 receptors function as dependence receptors to mediate oligodendrocyte cell death following contusive spinal cord injury.
MouseSpinal cord injuryContusionCD1 background: wild-type, EphB3 knockout, PLP-GFP wild-type and PLP-GFP-EphB3 knockout miceFemalen = ephrinB3 infusion n=6 versus vehicle n=14; PLP-GFP wild-typ…Intrathecal infusion into the injury epicentre via micro-osmotic pump (Alzet model 1007D) with Brain Infusion kit cannula100 micrograms/ml in the pump reservoir, delivered at 50 ng…Pump implanted at the time of surgery, immediately after laminectomy and contus…
Model: Contusive spinal cord injury: laminectomy at thoracic level 9 with Infinite Horizon impactor at 50 kDynes
Intrathecal ephrinB3 infusion increased surviving PLP-GFP oligodendrocytes at 7 days post injury (5870 plus or minus 742) versus vehicle (3547 plus or minus 510, P<0.05), but improved hindlimb locomotion was not observed. EphB3 knockout mice showed preserved oligodendrocyte numbers and myelin basic…
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