Grafting of a single donor myofibre promotes hypertrophy in dystrophic mouse muscle.
MouseOthermdx nude hosts; donors 3F-nlacZ-2E and beta-actin-Cre:R26NZGn = Per group n=4 to 10 across experiments, for example irradia…Intramuscular graft into tibialis anterior via fine glass needle4 microlitres of DMEM containing one myofibre per host musc…3 days after muscle injury
Model: Acute myotoxin injury of tibialis anterior by injection of 25 microlitres of 1.2 per cent barium chloride, or hindlimb irradiation with 18 Gy at 0.72 Gy/min with 10 microlitres of notexin at 10 micrograms/ml before grafting
A single grafted donor myofibre significantly increased host muscle weight and cross-sectional area by hypertrophy rather than hyperplasia, an effect not reproduced by barium chloride injury alone or by isolated satellite cells.
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