Poor Repair of Skeletal Muscle in Aging Mice Reflects a Defect in Local, Interleukin-33-Dependent Accumulation of Regulatory T Cells.
MouseOtherC57BL/6, including Foxp3-IRES-GFP, Kaede/B6, Foxp3-creYFP and ST2-flox lines; aged B6 from the NIA colonyMalen = Not reported as a single total; per-experiment groups of 4…Intramuscular or intraperitoneal0.3 ug per muscle intramuscularly, or 2 ug intraperitoneallyIntramuscular dose given at the time of injury; intraperitoneal dosing began th…
Model: Acute skeletal muscle injury induced by intramuscular cardiotoxin (0.03 ml per muscle at 0.03 mg/ml) into hindlimb muscles, with a milder cryoinjury model (liquid nitrogen chilled probe applied to tibialis anterior for 8 s) used for regeneration studies
IL-33 supplementation in aged mice restored muscle Treg accumulation, increased satellite cell representation and myogenic colony formation, and increased the number and mean cross-sectional area of centrally nucleated regenerating myofibres; deletion of ST2 on Tregs impaired regeneration.
Extraction: extract · Supplied extraction confidence: 0.9. Machine-extracted fields require source verification.