Tumor prevention facilitates delayed transplant of stem cell-derived motoneurons.
MouseOtherC57Bl/6n = Initial series of 17 transplants; mitomycin C-treated delay…Intraneural injection by glass micropipette into the transected distal tibial nerveTen thousand differentiated cells per transplant; mitomycin…Immediately after transection or after a delay of 1, 2, 4 or 8 weeks
Model: Tibial nerve transection at midthigh with ligation of both ends and burial of the proximal stump, producing prolonged denervation of the medial gastrocnemius
Mitomycin C pretreatment eliminated the residual pluripotent population and abolished teratocarcinoma formation (0 of 20 animals) while preserving motoneurons, giving twitch and tetanic forces after 1 to 4 week delayed transplantation similar to immediate transplantation of untreated cells, with no…
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