Acute Implantation of Aligned Hydrogel Tubes Supports Delayed Spinal Progenitor Implantation.
MouseSpinal cord injuryHemisection / partialC57BL/6J host mice; donor progenitors from embryonic day 14 EGFP-expressing C57BL/6 embryosFemale (host mice)n = n = 4 per condition for histology at week 4 (N = 16) and n…Intraspinal implantation of tubes into the hemisection defect and intraspinal injection of cells into the tubes or lesion epicentreFive hydrogel tubes implanted per injury; two intraspinal i…Tubes implanted immediately after the hemisection injury; spinal progenitors in…
Model: C5 lateral hemisection spinal cord injury created under 2% isoflurane after laminectomy, with gelfoam coverage; animals assessed to 4 weeks post-injury
At 2 weeks after transplantation (4 weeks after injury) 4.3% of transplanted E14 spinal progenitors survived when injected into tubes compared with 0.7% when injected into the injury alone, with a higher exogenous nestin-positive progenitor density (5.3 +/- 1.4 versus 1.1 +/- 0.3 cells/mm2, p = 0.0…
Extraction: extract · Subtype source: rule · Supplied extraction confidence: 0.85. Machine-extracted fields require source verification.