Toll-like receptor 5 deficiency diminishes doxorubicin-induced acute cardiotoxicity in mice.
MixedChemical/toxinGlobal TLR5 knockout mice on C57BL/6 background and age-matched wild-type littermates; AAV9-shNOX2 for NOX2 knockdownMale for flagellin experiments; not stated for remaining in vivo experimentsDoxorubicin intraperitoneal; AAV9 vectors intravenous; flagellin intravenous; route of neutralising antibody not statedTLR5 neutralising antibody 5 microgram per mouse in 100 mic…Neutralising antibody or isotype IgG given at the same time as doxorubicin; AAV…
Model: Doxorubicin-induced acute cardiotoxicity: single intraperitoneal doxorubicin 15 mg/kg with sacrifice at 3 days, plus a 7-day survival experiment; in vitro neonatal rat cardiomyocytes exposed to doxorubicin 1 microM for 24 h
TLR5 expression rose after doxorubicin. TLR5 knockout attenuated weight loss, preserved heart weight to tibia length and ejection fraction, lowered CK-MB and LDH, and reduced superoxide, 4-HNE, MDA, NADPH oxidase activity, NOX2, TNF-alpha, IL-1beta, NF-kappaB translocation, Bax and TUNEL positivity…
Extraction: extract · Supplied extraction confidence: 0.8. Machine-extracted fields require source verification.