Me6TREN targets beta-catenin signaling to stimulate intestinal stem cell regeneration after radiation.
MixedRadiationC57BL/6 primary; BALB/c confirmatory survival; Lgr5-EGFP-IRES-creERT2 for organoid and intestinal stem cell studiesn = Three or more mice per group for immunohistochemistry; n pe…Subcutaneous injection2.5 mg/kg identified as optimal; dose-response tested at 0,…First dose within 1 hour after radiation exposure (day 0), so a post-exposure m…
Model: Radiation-induced acute gastrointestinal syndrome: whole-body irradiation at 8 to 14 Gy using a cobalt-60 irradiator, and abdominal X-ray irradiation at 14 Gy; survival observed for 30 days. In vitro 6 Gy for organoids and 10 Gy for IEC-6 cells
Subcutaneous Me6TREN at 2.5 mg/kg on days 0, 3, 6, 9 and 12 improved survival after 8 Gy whole-body irradiation (40% versus 0% with PBS), extended median survival after 14 Gy from 5 to 10 days, and raised survival after 14 Gy abdominal irradiation to 80% versus 15%, with increased crypt and villus…
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