Circulating FH Protects Kidneys From Tubular Injury During Systemic Hemolysis.
MouseChemical/toxinC57BL/6 wild type and hepatocyte-specific Cfh knockout C57BL/6 (hepatoFH-/-, approximately 20% residual Factor H)n = 4 mice per experimental group, from one experimentintravenous per Methods; the Results text instead describes a single intraperitoneal injection250 microlitre of 1 mg/mL (0.25 mg) per injection, equivale…pre-treatment, 3 h and 1 h before phenylhydrazine
Model: Phenylhydrazine-induced acute systemic intravascular haemolysis, 900 micromol/kg (0.125 mg/g body weight) in 100 microlitre intraperitoneally as a single injection, with sacrifice at 6 h, day 1, day 2 or day 4; comparator injury models were LPS 2 mg/kg and cisplatin 15 mg/kg intraperitoneally
In hepatoFH-/- mice, phenylhydrazine raised blood urea at day 2 and this was prevented by pre-injection of purified human Factor H, which also partially prevented the rise in renal NGAL and Kim-1 and prevented systemic C3 cleavage. No effect was seen in wild type mice, in which tubular injury marke…
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