Inhibition of miRNA-34a Promotes M2 Macrophage Polarization and Improves LPS-Induced Lung Injury by Targeting Klf4.
MouseEndotoxin/LPSC57BL/6male or femalen = four mice per groupintraperitoneal2 mg/kg body weight in 50 microlitre sterile PBS (miR-34a m…given concurrently with intratracheal LPS instillation
Model: Lipopolysaccharide-induced acute lung injury; LPS from E. coli 055:B5, 10 microgram per mouse intratracheally under ketamine and xylazine anaesthesia
miR-34a was upregulated in LPS-instilled lungs and in LPS-treated RAW264.7 macrophages. The miR-34a inhibitor improved lung histology with reduced intra-alveolar PMN infiltration and MPO staining, restored Klf4 expression which had fallen by roughly 50-60% after LPS, reduced IL-1 beta positive cell…
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