Overexpression of TGFβ1 in murine mesenchymal stem cells improves lung inflammation by impacting the Th17/Treg balance in LPS-induced ARDS mice.
MouseEndotoxin/LPSC57BL/6 (wild-type); donor MSCs from C57BL/6 bone marrown = n = 3 per group at each time point for histology, western b…Intratracheal4 hours after lipopolysaccharide challenge
Model: Intratracheal lipopolysaccharide (Escherichia coli serotype 0111:B4, 2 mg/kg in 20 microlitres sterile normal saline) to induce acute respiratory distress syndrome
Compared with empty-vector MSCs, TGF-beta1-overexpressing MSCs further reduced the lung Th17/Treg ratio at days 3 and 7, decreased IL-17A and increased IL-10, lowered BALF total protein, albumin, TNF-alpha, IL-1beta and IL-6, increased occludin, and improved lung injury scores (all p < 0.05). Lung…
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