Targeting PKCtheta Promotes Satellite Cell Self-Renewal.
MouseCrushLimb / muscle crushPKCtheta knockout on C57BL/6J background with C57BL/6J wild-type controlsn = Reported as means of at least three experiments; per-group…Intraperitoneal in vivo; culture medium in vitro5 mg/kg in vivo; 0.5, 1 and 2 micromolar in vitroStarted 1 day before cardiotoxin injection
Model: Acute cardiotoxin injury: 20 microlitres of Naja pallida cardiotoxin given as two 10 microlitre injections into the gastrocnemius; a repeated injury protocol used three cardiotoxin injuries 20 days apart
Loss or pharmacological inhibition of PKCtheta increased symmetric self-renewing divisions via increased symmetric Pard3 distribution, expanding the quiescent satellite cell pool 28 days after cardiotoxin injury by 64.4 percent in knockout mice and about 50 percent with C20, and by 110 percent in k…
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