P2Y6 receptor inhibition aggravates ischemic brain injury by reducing microglial phagocytosis.
MouseIschaemia-reperfusionCerebral (MCAO/stroke)ICRMalen = Sham n = 6, saline plus DMSO n = 15, MRS2578 n = 12; figure…Intraperitoneal3 mg/kg, prepared from a 10 mg/mL DMSO stock diluted in sal…First injection 3 hours after occlusion
Model: Transient middle cerebral artery occlusion for 90 minutes with a silicone-coated 6-0 monofilament followed by reperfusion under isoflurane anaesthesia
Blocking P2Y6 with MRS2578 reduced microglial phagocytosis and worsened outcome: infarct volume, brain atrophy, neurological severity score, foot fault ratio, weight loss and mortality all increased. Inflammatory cytokines and MPO were unchanged. The findings indicate that endogenous P2Y6-mediated…
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