Intra-articular depletion of macrophages increases acute synovitis and alters macrophage polarity in the injured mouse knee.
MouseFractureTibiaMaFIA transgenic on C57BL/6 background, and wild-type C57BL/6Malen = 27 male MaFIA mice and 27 male C57BL/6 mice; per time point…Intra-articular injection into the knee joint spaceAP20187 60 micrograms (6 microlitre injection of 10 mg/mL);…Single injection either 2 days before fracture, immediately after fracture, or…
Model: Closed articular fracture of the left tibial plateau by controlled compression (10 N pre-load then 20 N/s to fracture, indenter displacement limited to 2.7 mm); right hind limb served as uninjured contralateral control
Intra-articular macrophage depletion did not reduce synovitis or synovial macrophage numbers 7 days after fracture. Depletion of MaFIA mice 2 days before fracture significantly increased synovitis (P = 0.003) and reduced tibial metaphyseal bone mineral density (P = 0.002 in the results text, P = 0.…
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