Evidence That the Anti-Inflammatory Effect of Rubiadin-1-methyl Ether Has an Immunomodulatory Context.
MouseEndotoxin/LPSSwissMalen = n = 6 per group in vivo; n = 3 per group for in vitro assay…Oral gavage in vivo; added to culture medium in vitroIn vivo 3, 10 and 30 mg/kg; in vitro 30 micromolar (cytotox…Pre-treatment given 1 h before intranasal LPS instillation in vivo; 0.5 h befor…
Model: LPS-induced acute lung injury: intranasal instillation of lipopolysaccharide 5 mg/kg in 50 microlitres PBS under xylazine/ketamine anaesthesia, with bronchoalveolar lavage fluid collected 12 h later; parallel in vitro model of RAW 264.7 macrophages stimulated with LPS 1 microgram/mL
In vivo, Rubiadin-1-methyl ether reduced total leukocyte counts at 10 and 30 mg/kg (43.6 and 55.3% inhibition) and neutrophil migration (48.1 and 68.8%), reduced exudation at all doses, reduced NOx (46.3 to 60.1%) and MPO activity (41.8 to 54.7%), reduced IL-12p70 and IL-6 at all doses, IFN-gamma a…
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