Transcriptomic analysis of alpha-synuclein knockdown after T3 spinal cord injury in rats.
RatSpinal cord injuryContusionSprague-Dawley (specific pathogen-free)Malen = 45 adult male Sprague-Dawley rats randomised into three gro…In situ intraspinal injection at the injury site10 microlitres of lentiviral vector at a titre of 1.0 x 10E…Immediately following the T3 spinal cord contusion
Model: T3 spinal cord contusion. The T3 lamina was removed to expose the spinal cord, which was struck once with a PSI-IH precision impactor at a strike strength of 400 kdyne with a compression time of 5 s. Sham operated animals had the spinal cord exposed only
Lentiviral shRNA significantly reduced alpha-synuclein protein and mRNA at the injury site. Body weight was higher in the treated group than the untreated injury group at day 28 and motor scores were higher at days 21 and 28. RNA sequencing identified 337 differentially expressed genes between inju…
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