Peripheral loss of EphA4 ameliorates TBI-induced neuroinflammation and tissue damage.
MouseTBIControlled cortical impactCD1 background; EphA4 floxed, Rosa, Cx3cr1-Cre and Tie2-Cre lines on C57BL/6 background bred onto CD1Malen = Seven mice pooled per group for CD45+ immune cell isolation…Subcutaneous Alzet mini-osmotic pump (Model 1007D), continuous infusion at 0.5 microlitre/h10 mg/kg/day of each peptidePumps implanted immediately following injury
Model: Controlled cortical impact, 4 mm craniotomy over right parietal-temporal cortex, 3 mm bevelled tip, 5.0 m/s, 2.0 mm depth, 100 ms dwell
Continuous infusion of VTM-EEKK or KYL at 10 mg/kg/day for 4 days after controlled cortical impact significantly reduced cortical lesion volume (2.00 +/- 0.26 mm and 1.97 +/- 0.43 mm versus 3.58 +/- 0.36 mm for vehicle), whereas the scrambled control peptide did not. Treatment lowered pro-inflammat…
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