Histone methyltransferase G9a protects against acute liver injury through GSTP1.
MouseEndotoxin/LPSC57BL/6, including liver-specific G9a-deficient (L-G9a) mice and littermate controlsmale for D-galactosamine/lipopolysaccharide and acetaminophen experiments; male and female for lipopolysaccharide-only injectionrGSTP1 intravenous; BIX intraperitonealrGSTP1 10 mg/kg; BIX 50 mg/kg body weightrGSTP1 given 30 min before lipopolysaccharide or acetaminophen injection; BIX g…
Model: D-galactosamine (200 mg/kg body weight, intraperitoneal) plus lipopolysaccharide (3 mg/kg body weight, intraperitoneal) induced acute liver injury; and acetaminophen overdose (400 mg/kg body weight, intraperitoneal, after overnight fasting)
Liver-specific G9a deletion caused 100 per cent mortality after lipopolysaccharide under D-galactosamine sensitisation and worsened acetaminophen-induced injury, and treatment with recombinant GSTP1 reversed the liver damage in G9a-deficient mice, identifying a protective G9a-GSTP1 axis.
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