Post-surgical inhibition of phosphatidylinositol 3-kinase attenuates the plantar incision-induced postoperative pain behavior via spinal Akt activation in male mice.
MouseWoundKunmingMale and female, analysed separatelyn = 224 male and 120 female Kunming mice, 344 in total; n per g…Intrathecal, into the subarachnoid space between L5 and L6 in conscious mice, 5 microL over 30 secondsWortmannin 0.016, 0.08 and 0.4 microg per 5 microL; LY29400…90 minutes after plantar incision for behavioural experiments; 45 minutes after…
Model: Plantar incision model of postoperative pain: under isoflurane a 5 mm longitudinal incision was made through the glabrous skin and fascia of the right hind paw, the flexor digitorum brevis muscle was elevated and divided, and the skin closed with a single mattress suture
Post-surgical intrathecal wortmannin or LY294002 dose-dependently attenuated mechanical allodynia, thermal hyperalgesia and cumulative pain scores in male mice, with only mild effects in female mice. In male mice the inhibitors partially reduced incision-induced spinal Fos expression and attenuated…
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