STAT6 induces expression of Gas6 in macrophages to clear apoptotic neutrophils and resolve inflammation.
MouseEndotoxin/LPSC57BL/6Male and femalen = n = 6 mice per time point for BALF ELISA and alveolar macro…Macrophages by non-invasive intratracheal instillation; LPS intraperitoneallyIn vitro priming with TSG6 400 ng/mL or IL-4 100 ng/mL for…Simultaneous, with animals receiving LPS intraperitoneally and primed macrophag…
Model: Lipopolysaccharide-induced acute lung injury: single intraperitoneal dose of LPS 10 mg/kg (Escherichia coli 0111:B4) for injury, permeability and BALF studies, and a lethal dose of 20 mg/kg intraperitoneally for mortality studies
Intratracheal transfer of TSG6- or IL-4-primed bone marrow-derived macrophages increased survival after lethal LPS, reduced lung vascular permeability, reduced BALF neutrophil accumulation and lowered proinflammatory cytokines compared with unstimulated macrophages. The benefit required Gas6, since…
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