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← Studies/PMID 31304988
PRECLINICAL PUBLICATION · 2019

Acute Changes in NADPH Oxidase 4 in Early Post-Traumatic Osteoarthritis.

Mouse / Other

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PMID 31304988 ↗2019Reported benefit

Acute Changes in NADPH Oxidase 4 in Early Post-Traumatic Osteoarthritis.

MouseOtherC57BL/6N wild-type and B6.129-Nox4 knockout miceFemalen = 80 mice used for gene expression across 10 post-injury time…Oral gavage in mice; added to culture medium in vitro20 mg/ml given daily by gavage in mice; 1 mM in primary hum…Started on day 0 immediately after injury

Model: Non-invasive tibial compression knee injury producing anterior cruciate ligament rupture by bony avulsion, delivered as a single dynamic overload at 1 mm/s to a target compressive force of 14 N, as a model of post-traumatic osteoarthritis; simulated injury in primary human chondrocytes using IL-1beta, TNF-alpha or TGF-beta at 10 ng/ml

Nox4 activity, mRNA and protein fell acutely within the first 24 h after joint injury in both human chondrocytes and mouse knees, then rose above baseline with a peak at 48 h and elevation persisting to 72 h. Daily GKT137831 for 7 days after injury prevented the significant fall in subchondral bone…

Extraction: extract · Supplied extraction confidence: 0.9. Machine-extracted fields require source verification.