TLR9 is essential for HMGB1-mediated post-myocardial infarction tissue repair through affecting apoptosis, cardiac healing, and angiogenesis
MouseIschaemia-reperfusionCardiacC57BL/6 wild-type and TLR9 knockout on a C57BL/6 backgroundMalen = Not fully reported in the retrieved text; CpG-ODN 1826 grou…Intramyocardial (left ventricular) injection for rhHMGB1; intraperitoneal injection for CpG-ODN 18262.5 ug rhHMGB1 in 100 ul PBS per mouse; CpG-ODN 1826 10 ug…1 h before left anterior descending artery ligation
Model: Permanent proximal left anterior descending coronary artery ligation with 7-0 silk via left hemithoracotomy to induce myocardial infarction, with sham-operated controls undergoing the same procedure without ligation
Intramyocardial rhHMGB1 given 1 h before ligation improved 28-day survival in wild-type mice (79.17% versus 52.17% for PBS-treated wild-type, log-rank p=0.0447), reduced cardiac rupture and infarct size, increased infarct wall thickness and collagen volume fraction, improved cardiac function, raise…
Extraction: extract · Subtype source: rule · Supplied extraction confidence: 0.85. Machine-extracted fields require source verification.