Agonist c-Met Monoclonal Antibody Augments the Proliferation of hiPSC-derived Hepatocyte-Like Cells and Improves Cell Transplantation Therapy for Liver Failure in Mice.
MouseChemical/toxinFRGS (Fah, Rag2 and Il2rg deficient, immunodeficient)n = 20 mice per group in survival studiesIntraperitoneal injection of antibody; splenic injection of cells0.5 mg/kg weekly in vivo; 0.5 mg/mL in culture with escalat…Cell transplantation at day 7 after NTBC withdrawal with 5D5 from weeks 1 to 8…
Model: Three models: tyrosinaemic stress by NTBC withdrawal; acute liver failure by repeated intraperitoneal JO2 Fas/CD95 agonistic antibody every three days; chronic injury by carbon tetrachloride twice weekly for six weeks
Adding 5D5 to hepatocyte-like cell transplantation raised human liver chimerism to over 40 per cent and improved survival and liver function across the Fas, tyrosinaemic and carbon tetrachloride injury models without tumour formation.
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