The skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells.
MouseWoundC57BL/6Both male and femalen = Untreated control n = 12, carrageenan n = 9, carrageenan pl…Topical application to the wound, alone or embedded in a 6 mm hydrogel discApproximately 100 microlitres of conditioned medium equival…Applied at the time of wounding
Model: Full-thickness 6 mm dorsal excisional skin wound made with a sterile biopsy punch under ketamine (100 mg/kg) and xylazine (10 mg/kg) anaesthesia, dressed with transparent film and a sutured silicone ring
The secretome stimulated capillary-like tube formation in HUVEC cultures and significantly increased blood vessel density in wound granulation tissue at 14 days, both alone and when combined with carrageenan. It did not improve wound closure, granulation tissue thickness, or epidermal thickness and…
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