Acute tobacco smoke exposure exacerbates the inflammatory response to corneal wounds in mice via the sympathetic nervous system.
MouseWoundC57BL/6J and a knock-in Cys to Ala gene-mutated line on C57BL/6J backgroundFemale6-hydroxydopamine and SB 225002 intraperitoneal; timolol, isoepinephrine, tamsulosin and antibodies topical to the eye6-hydroxydopamine 100 mg/kg; timolol 5 microlitres of 0.01…6-hydroxydopamine on days -7, -5 and -3 before abrasion; adrenergic drugs appli…
Model: Central corneal epithelial abrasion (2 mm diameter marked with a trephine and scraped with a microspud) combined with acute tobacco smoke exposure (approximately 3 cigarettes over 90 min in a ventilated chamber)
Acute tobacco smoke exposure delayed corneal re-epithelialisation and amplified neutrophil and gamma delta T cell influx with raised NF-kappaB, IL-6 and IL-17 expression, and sympathectomy, adrenergic receptor blockade and IL-6 or IL-17A neutralisation each independently attenuated these effects.
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