Sonic hedgehog signaling is negatively regulated in reactive astrocytes after forebrain stab injury.
MouseTBIPenetrating / ballisticTransgenic reporter and Smoothened conditional knockout lines with wild type controlsMale and femalen = At least 3 animals per group; CD45 data from 3 to 4 animals…Direct intracortical infusion via an Alzet 1007D miniosmotic pump with a cannula inserted 2 mm into the lesioned cortex1 micromolar in 0.9% saline in the pump reservoirPump implanted immediately after stab injury
Model: Forebrain stab injury through a 3 by 3 mm craniotomy 1 mm caudal and lateral to bregma, with a number 11 scalpel blade inserted 3 mm and moved rostral to caudal three times
SAG infusion reduced CD45 positive leukocytes by 74% compared with vehicle (9.25 plus or minus 1.4 versus 36.04 plus or minus 9.8) without changing proliferation, and this anti-inflammatory effect was abolished in Smoothened conditional knockout mice, indicating an astrocyte-mediated neuroprotectiv…
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