Inhibition of multiple voltage-gated calcium channels may contribute to spinally mediated analgesia by epipregnanolone in a rat model of surgical paw incision.
RatWoundSprague-DawleyFemale for in vivo experiments; both sexes for dorsal root ganglion recordingsn = 51 dorsal root ganglion cells from 28 rats; behavioural gro…Intrathecal, 50 ul via a 28 G needle into the lumbar region50 ul of 300 uM in the incision model; 3, 100 and 300 uM ea…24 h after surgical incision
Model: Plantar incision of the hind paw, with longitudinal incision of skin and plantaris muscle under isoflurane anaesthesia (postoperative pain model)
A single intrathecal injection of 300 uM epipregnanolone roughly doubled mechanical withdrawal thresholds in incised rats over 120 min compared with vehicle (p = 0.0038) and produced dose-dependent thermal and mechanical antinociception in healthy rats without sensorimotor impairment.
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