Notch inhibitor can attenuate apparent diffusion coefficient and improve neurological function through downregulating NOX2-ROS in severe traumatic brain injury.
RatTBIWeight-drop / impact-accelerationSprague-Dawley (specific pathogen free)n = 50 rats, n=10 per group across 5 groupsIntracerebroventricular stereotactic injection into the right lateral ventricle (anteroposterior minus 0.8 mm, mediolateral 1.6 mm, dorsoventral minus 4.0 mm from bregma)DAPT 0.03 mg per kg body weight (prepared at 1 microgram pe…Half an hour after injury
Model: Weight-drop craniocerebral injury under 1 percent pentobarbital 30 mg per kg intraperitoneally; a 5 mm diameter bone window drilled, dura exposed, and a 40 g hammer dropped vertically from 25 cm to produce moderate to severe traumatic brain injury; animals killed 24 hours after injury
DAPT significantly reduced apparent diffusion coefficient values and neurological severity scores and downregulated NICD, Hes1, Hes5 and NOX2 with reduced reactive oxygen species, although cerebral blood flow was unchanged.
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