Mesenchymal stromal cell potency to treat acute kidney injury increased by ultrasound-activated interferon-gamma/interleukin-10 axis.
MouseChemical/toxinC3H wild-type and B6.129S7 interferon-gamma knockoutFemalen = n=6 mice per group across all experimental groupsIntravenous tail vein infusion of MSC; transcutaneous image-guided pulsed focused ultrasound to the kidneysMSC infused in 100 microl per mouse (exact cell number not…pFUS delivered 24 h after cisplatin and MSC infused approximately 4 h after pFUS
Model: Cisplatin-induced acute kidney injury by a single intraperitoneal injection of cisplatin 15 mg/kg on day 0, with food and water withheld for 12 h beforehand
Interferon-gamma preconditioning increased MSC IL-10 in vitro and produced small but significant improvements in BUN and serum creatinine, fewer TUNEL-positive cells and a trend to lower KIM-1 versus unstimulated MSC, without changing MSC homing. In interferon-gamma knockout mice, pFUS nearly doubl…
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