The novel estrogenic receptor GPR30 alleviates ischemic injury by inhibiting TLR4-mediated microglial inflammation.
MouseIschaemia-reperfusionCerebral (MCAO/stroke)C57BL/6Female, ovariectomisedn = 170 mice in total, per-group numbers not stated17-beta-oestradiol intraperitoneal; G1 and ICI182780 by left intracerebroventricular infusion at 1 microlitre per minute17-beta-oestradiol 235 microlitres at 1 mmol/l; G1 2 microl…Immediately upon reperfusion, that is 1 h after onset of occlusion
Model: Bilateral ovariectomy followed one week later by intraluminal filament middle cerebral artery occlusion for 1 h with reperfusion, with laser Doppler confirmation of flow reduction and restoration
G1 and 17-beta-oestradiol significantly reduced infarct volume (p=0.0010 for G1 and p<0.0001 for oestradiol versus vehicle) and neurological deficit (p=0.0008 and p=0.0002), with fewer TUNEL positive and more NeuN positive neurons and lower penumbral cytokines.
Extraction: extract · Subtype source: rule · Supplied extraction confidence: 0.85. Machine-extracted fields require source verification.