Assessment of systemic administration of PEGylated IGF-1 in a mouse model of traumatic brain injury.
MouseTBIControlled cortical impactC57BL/6JMalen = 6 to 8 per group in the 24 h study; 6 injured and 3 sham pe…Subcutaneous injection0.5, 1 or 3 mg/kg (diluted to 0.05, 0.1 or 0.3 mg/ml in PBS)First dose 3 h after controlled cortical impact
Model: Controlled cortical impact over the left hemisphere, 5 mm craniotomy, 3 mm rounded tip, 1 mm depth, 3.5 m/s velocity, producing a moderate to severe cortical contusion
Subcutaneous PEG-IGF-1 produced dose-dependent increases in serum concentrations but did not alter cortical contusion volume (ANOVA F(2,18) = 1.567, P = 0.24), numbers of Fluorojade C-positive degenerating dentate granule neurons (F(2,17) = 0.1305, P = 0.88), or recovery of doublecortin-positive im…
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