Inhibition of 2-AG hydrolysis differentially regulates blood brain barrier permeability after injury.
MouseEndotoxin/LPSCD1 (lipopolysaccharide model and pharmacokinetics); C57BL/6 (photothrombosis model)Male (CD1 lipopolysaccharide model)Subcutaneous10 mg/kg in 5:5:90 dimethyl sulfoxide:Cremophor:salineLipopolysaccharide model: 30-45 min after each lipopolysaccharide dose. Ischaem…
Model: Two models. Systemic inflammation with Salmonella enterica typhimurium lipopolysaccharide 3 mg/kg intraperitoneally at 0, 6 and 24 h; and focal photothrombotic cortical ischaemia by retro-orbital rose bengal (30 uL of 15 mg/mL) with 10 min green laser illumination over somatosensory cortex
CPD-4645 raised brain 2-arachidonoylglycerol, lowered arachidonic acid, IL-1beta and IL-6, reversed 74% of lipopolysaccharide-induced vascular gene induction and reduced barrier leakage in both models; CB1 and CB2 blockade partially reversed only the ischaemic protection.
Extraction: extract · Supplied extraction confidence: 0.82. Machine-extracted fields require source verification.