Novel mechanisms of Collagenase Santyl Ointment (CSO) in wound macrophage polarization and resolution of wound inflammation.
MouseWoundC57BL/6 and BKS.Cg-m +/+ Leprdb/J (db/db)MaleTopical, loaded into implanted subcutaneous PVA sponges300 mg CSO per sponge; CS-API 250 ng/mL in vitroLoaded into sponges at the time of implantation, that is at wounding
Model: Subcutaneous implantation of 8 mm sterile PVA sponges with sutured incisional wounds; acute wound model in C57BL/6 and chronic diabetic wound model in db/db mice
CSO and CS-API shifted wound macrophages toward a pro-reparative phenotype with higher IL-10 and TGF-beta and lower TNF-alpha and IL-1beta in both acute and diabetic wounds, acting through PGE2-EP4-dependent STAT6 activation.
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