Inhibition of microRNA-210 suppresses pro-inflammatory response and reduces acute brain injury of ischemic stroke in mice.
MouseIschaemia-reperfusionCerebral (MCAO/stroke)C57BL/6Malen = 85 mice purchased with 11 added for dropouts, 96 used overa…Intracerebroventricular injection into the left lateral ventricle100 pmol in 4 ul24 h before MCAO (pretreatment) or 4 h after MCAO (post-treatment)
Model: Focal cerebral ischaemia by intraluminal middle cerebral artery occlusion for 60 min followed by reperfusion, under 1.5% isoflurane with body temperature at 37 +/- 0.5 degrees C
Pretreatment with miR-210-LNA abolished the ischaemia-induced rise in brain miR-210 and reduced infarct volume (30.6 +/- 1.5% versus 49.2 +/- 2.9%, p < 0.01) and oedema, with improved neurological score and foot-fault performance. It suppressed TNF-alpha, IL-1beta, IL-6, CCL2 and CCL3 and reduced m…
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