Pharmacological modulation of C-X-C motif chemokine receptor 4 influences development of acute respiratory distress syndrome after lung ischaemia-reperfusion injury.
RatIschaemia-reperfusionOther / unspecifiedSprague-DawleyMalen = 27 rats: series 1 vehicle n = 5 and AMD3100 n = 5; series 2…Intra-arterial (femoral artery)AMD3100 0.7 micromol/kg (700 nmol/kg) in 0.5 mL normal sali…Within 5 minutes of the onset of reperfusion
Model: Unilateral warm lung ischaemia-reperfusion under isoflurane anaesthesia and mechanical ventilation: right lateral thoracotomy with suture ligation of the right lung hilum occluding pulmonary artery, vein and right main stem bronchus for 30 minutes (series 1) or 60 minutes (series 2), followed by 300 minutes of reperfusion
CXCR4 blockade with AMD3100 worsened outcome, reducing PaO2/FiO2 ratios, converting mild into moderate ARDS, increasing histological lung injury and lowering lung IL-10, whereas the CXCR4 agonist ubiquitin dose-dependently increased PaO2/FiO2 ratios, converted moderate to severe into mild to modera…
Extraction: extract · Supplied extraction confidence: 0.95. Machine-extracted fields require source verification.