Intracellular calcium release through IP3R or RyR contributes to secondary axonal degeneration.
MouseSpinal cord injuryHemisection / partialYFP-expressing transgenic mice (Thy1-YFP reporter line)Dissolved in artificial cerebrospinal fluid and applied by continuous bath perfusion of the ex vivo preparationApplied 1 hour after injury and perfused continuously for the remainder of the…
Model: Highly focal laser-induced spinal cord injury (LiSCI) in an ex vivo preparation, with ascending dorsal column axons severed by an 800 nm laser pulse during two-photon time-lapse imaging
Distal axonal segments retracted significantly further than proximal segments by 4 hours after laser injury. Pharmacological or genetic targeting of either the ryanodine receptor or the IP3 receptor significantly reduced proximal axonal dieback and bystander secondary degeneration at 6 hours compar…
Extraction: extract · Subtype source: llm · Supplied extraction confidence: 0.65. Machine-extracted fields require source verification.