Bioengineered constructs combined with exercise enhance stem cell-mediated treatment of volumetric muscle loss.
MixedOtherRecipients NOD.CB17-Prkdcscid/J (NOD-SCID) or NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG); donors C57BL/6, ROSA26, FVB-Tg(CAG-luc,-GFP)L2G85Chco/J and Pax7-Cre; scaffolds from C57BL/6Male C57BL/6, ROSA26 and FVB luciferase or GFP donors; female NOD/MrkBomTac micen = Acute volumetric muscle loss transplantation n = 6; chronic…Surgical implantation into the tibialis anterior defect, sutured proximally and distally with 10-0 sutures150,000 muscle stem cells alone, or 150,000 muscle stem cel…Acute model transplanted immediately after ablation; chronic model transplanted…
Model: Volumetric muscle loss created by excising a 2 x 7 x 2 mm piece of the tibialis anterior muscle using a stencil and scalpel, giving approximately 40% ablation and an excised mass of 15 plus or minus 1.5 mg. The chronic model involved scar debridement 30 days after injury.
Bioconstructs carrying muscle stem cells plus muscle resident cells restored donor-derived myofibre formation, vascularisation, muscle mass and ex vivo force in acute and chronic volumetric muscle loss, yielding 413 plus or minus 87 donor myofibres versus 92 plus or minus 21 for stem cells alone an…
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