Acute administration of catalase targeted to ICAM-1 attenuates neuropathology in experimental traumatic brain injury.
MouseTBIControlled cortical impactC57BL/6J; CX3CR1-GFP (B6.129P-Cx3cr1tm1Litt/J) for two-photon microglial imagingMalen = n = 4 per condition for hydrogen peroxide assay; n = 3 mice…Intravenous, by retro-orbital injection100 ug anti-ICAM-1/catalase conjugate per mouse; comparator…30 min after controlled cortical impact
Model: Moderate controlled cortical impact traumatic brain injury: 4 mm craniectomy over right somatosensory cortex, impact at 3.5 m/s to 1 mm depth with a 2 mm piston and 0.5 s dwell time
Targeted anti-ICAM-1/catalase given 30 min post-impact normalised cortical hydrogen peroxide, cut 3-nitrotyrosine, preserved blood-brain barrier integrity and tight junction proteins, and reduced neuronal loss, astrogliosis and microglial activation. Non-targeted catalase or anti-ICAM-1 antibody al…
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