Targeted siRNA delivery reduces nitric oxide mediated cell death after spinal cord injury.
MouseSpinal cord injuryCompressionBALB/c in vivo; C57BL/6 bone marrow-derived macrophages in vitroFemaleIntraspinal injection into the injured cord via Hamilton syringe10 micrograms siRNA in 10 microlitres of chitosan nanoparti…Intraoperatively, after compression and before wound closure; exact interval no…
Model: Compression spinal cord injury: bilateral hemi-laminectomy at the low thoracic region with compression forceps deforming the cord to 0.15 mm, approximately 10 per cent of original cord width
Antibody-targeted siRNA chitosan nanoparticles reduced inducible nitric oxide synthase and Bax by approximately 50 per cent and roughly doubled Bcl-2 in the injured cord at 24 hours.
Extraction: extract · Subtype source: rule · Supplied extraction confidence: 0.8. Machine-extracted fields require source verification.