Role of carbonyl sulfide in acute lung injury following limb ischemia/reperfusion in rats.
RatIschaemia-reperfusionLimb / skeletal muscleSprague-Dawley, pathogen freeMalen = 64 rats in total, randomly divided into 8 groups of 8 anima…Intraperitoneal0.2 ml, 0.5 ml or 1.0 ml of 1:1 COS in air; air control vol…20 min before reperfusion, during the 4 h ischaemic period
Model: Bilateral hind limb ischaemia by rubber tourniquet at the limb root for 4 h followed by 2 h reperfusion, producing acute lung injury; anaesthesia with sodium pentobarbital 40 mg/kg intraperitoneally
Low dose carbonyl sulfide significantly reduced histological damage, lung coefficient, ICAM-1 and plasma and lung IL-1beta, IL-6 and TNF-alpha (p less than 0.01), and lowered the apoptotic index from 17.2% with injury alone to 5.83%, 6.48% and 7.88% at 0.2, 0.5 and 1.0 ml. Higher doses did not redu…
Extraction: extract · Subtype source: rule · Supplied extraction confidence: 0.9. Machine-extracted fields require source verification.