Regenerative Responses and Axon Pathfinding of Retinal Ganglion Cells in Chronically Injured Mice.
MouseCrushPeripheral-nerve crushC57BL/6N wild-type and Bax knockout (B6.129X1, Jackson stock 002994)male and femalen = n = 5 per group for RGC survival; n = 3 for acute AAV-CNTF…intravitrealAAV2 titre reported as 1 to 4 x 10^n particles per millilit…immediately after crush or 56 days after crush
Model: Intraorbital optic nerve crush with jeweller's forceps (Dumont no. 5) for 10 seconds approximately 1 mm behind the optic disc
AAV-CNTF delivered 56 days after crush produced regenerating axons beyond 1 mm in 5 of 5 mice versus none with AAV-GFP, with fewer misguided axons than acute treatment (2.7 +/- 2.3 per cent versus 14.7 +/- 2.1 per cent, P = 0.0029), despite retinal ganglion cell survival falling to 4.0 +/- 0.3 per…
Extraction: extract · Subtype source: rule · Supplied extraction confidence: 0.9. Machine-extracted fields require source verification.