Nerve growth factor delivery by ultrasound-mediated nanobubble destruction as a treatment for acute spinal cord injury in rats.
RatSpinal cord injuryContusionSprague-Dawleymaleintravenous via tail vein, with ultrasound applied over the injury siteFormulation only: plasmid 1 mg/mL added to 2 mL chloroform…immediately after acute spinal cord injury
Model: Modified Allen weight-drop contusion at the tenth thoracic segment, 10 g rod dropped from 5 cm and left resting for 3 minutes after laminectomy
NGF/PLGA nanobubbles with ultrasound gave the highest NGF mRNA and protein expression (P < 0.05), preserved Nissl bodies (P < 0.01), reduced TUNEL-positive cells (P < 0.01) and higher BBB scores from day 7 onward compared with all three control groups.
Extraction: extract · Subtype source: rule · Supplied extraction confidence: 0.85. Machine-extracted fields require source verification.