Activation of E-prostanoid 3 receptor in macrophages facilitates cardiac healing after myocardial infarction
MouseIschaemia-reperfusionCardiacC57BL/6 background; Ep3 floxed, myeloid-specific Ep3 knockout (LysM-Cre), global Ep3 knockout and CD68 promoter-driven macrophage-specific Ep3alpha transgenic linesmale and female
Model: Permanent left anterior descending coronary artery ligation to induce myocardial infarction; zymosan-induced peritonitis used as a companion sterile inflammation model
Ep3 deficiency in monocytes and macrophages reduced Ly6C-low cell accumulation in infarcted hearts from day 4, lowered CX3CR1 and VEGF expression by roughly half, reduced peri-infarct neovascularisation at day 14, expanded infarct area and impaired recovery of cardiac function. Wild-type bone marro…
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